mouse anti parvalbumin pv antibody  (Swant)

Journal: Neuropharmacology

Article Title: Estrogenic regulation of perineuronal nets in the mouse insular cortex and hippocampus

doi: 10.1016/j.neuropharm.2025.110641

Figure Lengend Snippet: Effects of estrous cycle stage on perineuronal net (PNN) and parvalbumin (PV) fluorescence staining intensities in the insula. Brain sections containing the anterior insula from mice in each stage of the estrus cycle were stained with fluorescently-labeled Wisteria floribunda agglutinin (WFA) for PNNs and an antibody to PV. A Representative image of the anterior insula at 10X magnification from a mouse in estrus. WFA is shown in green and PV in magenta. Scalebar, 0.3 mm. B 20X magnification of area outlined by box in A . Scalebar, 0.125 mm. C Number of WFA + cells per 20X z-stack image. D Number of PV + cells per 20X z-stack image. E WFA intensity in each mouse relative to mean WFA intensity of mice in estrus. F Violin plots of relative WFA intensity for all cells (n = 772 in estrus, 437 in metestrus, 831 in diestrus, and 581 in proestrus). G PV intensity in each mouse relative to mean PV intensity of mice in estrus. H Violin plots of relative PV intensity for all cells (n = 559 in estrus, 326 in metestrus, 561 in diestrus, and 405 in proestrus). I Percentage of PV + cells that are also WFA+. In C , D , E , G , and I , data are shown as the mean ± SEM, n = 6 mice per stage. * p < 0.05, **** p < 0.0001 by Dunnett’s T3 multiple comparisons test. In F and H , the solid line on the violin plot is the median and dashed lines are quartiles. **** p < 0.0001 by Dunn’s multiple comparisons test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Mouse anti-parvalbumin (PV) antibody (#195011) was purchased from Synaptic Systems (Goettingen, Germany).

Techniques: Fluorescence, Staining, Labeling

Journal: Neuropharmacology

Article Title: Estrogenic regulation of perineuronal nets in the mouse insular cortex and hippocampus

doi: 10.1016/j.neuropharm.2025.110641

Figure Lengend Snippet: Effects of estrous cycle stage on perineuronal net (PNN) and parvalbumin (PV) fluorescence staining intensities in the hippocampus. Brain sections containing the dorsal CA1 region of the hippocampus from mice in each stage of the estrus cycle were stained with fluorescently-labeled Wisteria floribunda agglutinin (WFA) for PNNs and an antibody to PV. A Representative image of the stratum oriens and CA1 region at 10X magnification from a mouse in estrus. WFA is shown in green and PV in magenta. Scalebar, 0.3 mm. B 20X magnification of area outlined by box in A . Scalebar, 0.125 mm. C Number of WFA + cells per 10X image. D Number of PV + cells per 10X image. E WFA intensity in each mouse relative to mean WFA intensity of mice in estrus. F Violin plots of relative WFA intensity for all cells (n = 322 in estrus, 251 in metestrus, 301 in diestrus, and 296 in proestrus). G PV intensity in each mouse relative to mean PV intensity of mice in estrus. H Violin plots of relative PV intensity for all cells (n = 196 in estrus, 179 in metestrus, 167 in diestrus, 147 in proestrus). I Percentage of PV + cells that are also WFA+. In C , D , E , G , and I , data are shown as the mean ± SEM, n = 5–6 mice per stage. In F and H , the solid line on the violin plot is the median and dashed lines are quartiles. **** p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05 by Dunn’s multiple comparisons test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Mouse anti-parvalbumin (PV) antibody (#195011) was purchased from Synaptic Systems (Goettingen, Germany).

Techniques: Fluorescence, Staining, Labeling

Journal: Neuropharmacology

Article Title: Estrogenic regulation of perineuronal nets in the mouse insular cortex and hippocampus

doi: 10.1016/j.neuropharm.2025.110641

Figure Lengend Snippet: Estrogen receptor and aromatase expression in parvalbumin neurons in the insula and hippocampus. RNAScope fluorescence in situ hybridization was performed on brain sections containing the anterior insula and dorsal hippocampal CA1 region with probes to Esr1, Esr2 , Pvalb , and Cyp19a1 . A Representative 40X image of the insula probed for Esr1 (magenta), Esr2 (white), and Pvalb (green) from a mouse in estrus. Yellow arrow points to a Pvalb -expressing cell that contains Esr2 transcripts; salmon arrow points to a Pvalb -expressing cell that contains Esr1 and Esr2 transcripts. Scalebar, 50 μm. B Percentages of Pvalb -expressing cells in the insula containing transcripts for Esr1 , Esr2 , both Esr1 and Esr2 , or neither Esr1 nor Esr2 . (estrus vs. diestrus, Fisher’s exact test, p = 0.028). C Percentages of Pvalb -expressing cells in the dorsal hippocampal CA1 region containing transcripts for Esr1 , Esr2 , both Esr1 and Esr2 , or neither Esr1 nor Esr2 . D Representative 40X image of the insula probed for Esr1 (magenta), Cyp19a1 (white), and Pvalb (green) from a mouse in estrus. Orange arrow points to a Pvalb -expressing cell that contains Cyp19a1 transcripts; Lilac arrow points to a Pvalb -expressing cell that contains Cyp19a1 and Esr1 transcripts. Scalebar, 50 μm. E Percentages of Pvalb -expressing cells in the insula containing transcripts for Esr1 , Cyp19a1 , both Esr1 and Cyp19a1 , or neither Esr1 nor Cyp19a1 . F Percentages of Pvalb -expressing cells in the dorsal hippocampal CA1 region containing transcripts for Esr1 , Cyp19a1 , both Esr1 and Cyp19a1 , or neither Esr1 nor Cyp19a1 (estrus vs. diestrus, Fisher’s exact test, p = 0.048). Data were obtained from 4 mice in estrus and 4 in diestrus for dHPC; 4 in estrus and 3 in diestrus for insula, for a total of 12 images from mice in estrus and 9–12 images from mice in diestrus for each brain region. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Mouse anti-parvalbumin (PV) antibody (#195011) was purchased from Synaptic Systems (Goettingen, Germany).

Techniques: Expressing, RNAscope, Fluorescence, In Situ Hybridization

Journal: Neuropharmacology

Article Title: Estrogenic regulation of perineuronal nets in the mouse insular cortex and hippocampus

doi: 10.1016/j.neuropharm.2025.110641

Figure Lengend Snippet: Effects of estradiol on perineuronal net (PNN) and parvalbumin (PV) fluorescence staining intensities in the insula and hippocampus. Ovariectomized mice were treated with estradiol benzoate (EB) or vehicle (VEH). Brain sections containing the anterior insula ( A-G ) and dorsal hippocampal CA1 (dHPC) region ( H-N ) were stained with fluorescently-labeled Wisteria floribunda agglutinin (WFA) for PNNs and an antibody to PV. A, H Number of WFA + cells per 20X ( A ) or 10X ( H ) image. B, I Number of PV + cells per 20X ( A ) or 10X ( I ) image. C, J Percentage of PV + cells that are WFA+. D, K WFA intensity in each mouse relative to mean WFA intensity of VEH-treated mice. E, L Relative WFA intensity for all cells ( E , insula, n = 1618 VEH and 1663 EB; L , dHPC, n = 1802 VEH and 2894 EB). F, M PV intensity in each mouse relative to mean WFA intensity of VEH-treated mice. G, N Relative PV intensity for all cells ( G , insula, n = 1217 VEH and 1229 EB; N , dHPC, n = 1613 VEH and 2007 EB). In A-D , F , H-K , and M , data are shown as mean ± SEM, n = 8 VEH- and 11 EB-treated mice. *p < 0.05, **p < 0.01 by Welch’s t -test. In the violin plots in E , G , L , and N , the solid line on the violin plot is the median and dashed lines are quartiles. *p < 0.05, ****p < 0.0001 by Mann-Whitney test.

Article Snippet: Mouse anti-parvalbumin (PV) antibody (#195011) was purchased from Synaptic Systems (Goettingen, Germany).

Techniques: Fluorescence, Staining, Labeling, MANN-WHITNEY

Journal: Neuropharmacology

Article Title: Estrogenic regulation of perineuronal nets in the mouse insular cortex and hippocampus

doi: 10.1016/j.neuropharm.2025.110641

Figure Lengend Snippet: Effects of letrozole on perineuronal net (PNN) and parvalbumin (PV) fluorescence staining intensity in the insula and hippocampus. Ovariectomized mice were treated chronically with the aromatase inhibitor letrozole (LTZ) or vehicle (VEH). Brain sections containing the anterior insula ( A-G ) and dorsal hippocampal CA1 (dHPC) region ( H-N ) were stained with fluorescently-labeled Wisteria floribunda agglutinin (WFA) for PNNs and an antibody to PV. A, H Number of WFA + cells per 20X ( A ) or 10X ( H ) image. B, I Number of PV + cells per 20X ( B ) or 10X ( I ) image. C, J Percentage of PV + cells that are WFA+. D, K WFA intensity in each mouse relative to mean WFA intensity of VEH-treated mice. E, L Relative WFA intensity for all cells ( E , insula, n = 2352 VEH and 2161 LTZ; L , dHPC, n = 690 VEH and 733 LTZ). F, M PV intensity in each mouse relative to mean WFA intensity of VEH-treated mice. G, N Relative PV intensity for all cells ( G , insula, n = 1842 VEH and 1404 LTZ; N , dHPC, n = 619 VEH and 497 LTZ). In A-D , F , H-K , and M , data are shown as mean ± SEM, n = 9 VEH for insula, n = 8 VEH for dHPC and n = 8 LTZ. *p < 0.05 by Welch’s t -test. In the violin plots in E , G , L , and N , the solid line on the violin plot is the median and dashed lines are quartiles. ****p < 0.0001 by Mann-Whitney test.

Article Snippet: Mouse anti-parvalbumin (PV) antibody (#195011) was purchased from Synaptic Systems (Goettingen, Germany).

Techniques: Fluorescence, Staining, Labeling, MANN-WHITNEY

Journal: Current Research in Neurobiology

Article Title: Regulation of perisomatic synapses from cholecystokinin basket interneurons through NrCAM and Ankyrin B

doi: 10.1016/j.crneur.2025.100150

Figure Lengend Snippet: Perisomatic synaptic puncta from PV-BCs onto PN soma are not decreased in mPFC of NrCAM-null mutant mPFC. A. Immunofluorescent staining of Syt2B + synaptic puncta (red, arrowheads) on MATH2+ PN soma (blue) in WT and NrCAM-null mPFC layer 2/3. Confocal images were captured with a 63X objective in a field of view of 2048× 2048 pixels, or 102 μm × 102 μm. The mean numbers of soma per image were: WT 10 ± 3, NrCAM-null 10 ± 2. Representative regions of images shown were obtained from single optical sections (∼0.3 μm) (bars = 20 μm). B. Quantitation of Syt2b + synaptic puncta per MATH2+ soma (x10 −1 ). Data points represent the number of puncta per soma in each image. Mean numbers of Syt2b + puncta per MATH2+ soma between genotypes WT and NrCAM-null were not significant (ns) two tailed t -test: p = 0.08. C. Immunostaining of VGLUT3+ CCK-BC synaptic puncta (red, arrowheads) on PV-BC soma immunostained for parvalbumin (blue). Confocal images were captured with a 40X objective in a field of view of 512× 512 pixels, or 160 μm × 160 μm. The mean numbers of soma per image were: WT 6 ± 1, NrCAM-null 7 ± 3. Representative regions of images shown were obtained from maximum intensity projections of z-stacks, 5 optical sections (∼0.3 μm each) (bars = 20 μm). D. Quantitation of VGLUT3+ synaptic puncta on PV-BC soma stained for parvalbumin. Data points represent the number of puncta per soma per image. Mean numbers of VGLUT3+ puncta per PV + soma were not significantly different (ns) between WT and NrCAM-null genotypes (two tailed t -test: p = 0.28).

Article Snippet: Primary antibody solutions contained 1:100 mouse anti-VGLUT3 antibody (Synaptic Systems 135211, 1 mg/ml) and 1:100 rabbit anti-Math2/NeuroD6 antibody (Abcam 85824, 0.9 mg/ml); 1:500 mouse anti-Parvalbumin (PV) 3C9 antibody (Novus Biologicals NBP2-50038, 1 mg/ml) and 1:500 rabbit anti-VGLUT3 antibody (Synaptic Systems 135203, 1 mg/ml); or 1:2500 rabbit anti-NECAB1 antibody (Sigma HPA023629, 0.20 mg/ml).

Techniques: Mutagenesis, Staining, Quantitation Assay, Two Tailed Test, Immunostaining

Journal: Current Research in Neurobiology

Article Title: Regulation of perisomatic synapses from cholecystokinin basket interneurons through NrCAM and Ankyrin B

doi: 10.1016/j.crneur.2025.100150

Figure Lengend Snippet: Expression of tdTomato, NECAB1, and Parvalbumin in mPFC of Sncg-IRES-FlpO:tdT mice (P60) A. Immunofluorescent staining of tdT with dsRed antibodies in a representative confocal image (single optical section) captured at 20x. tdT labeled cells were present throughout the mPFC with greater abundance in layer II/III. Cortical layers I-VI are indicated. Image field of view was 1408 x 1408 pixels, or 3904 μm × 3904 μm. B. Control staining with secondary antibodies alone was low. C. Higher magnification of tdT + cells show neuronal morphology with labeled soma and processes (arrowheads). Bar = 10 μm. D. Intrinsic tdT fluorescence in mPFC layer 2/3 neurons without dsRed antibody staining is lower but visible in soma and processes (arrowheads). E. Time course of tdT expression at postnatal ages P34 (a), P42 (b), and P60 (c, d). Representative nonconfocal images of mPFC layer II/III stained for tdTomato using dsRed antibodies, or secondary antibodies alone, were obtained by EVOS microscopy. Images were captured with a 20X objective in a field of view of 2048× 1536 pixels, or 633 μm × 475 μm. F. Graph of cell density per mm2 by postnatal age. Significant differences in mean cell density∗ were determined by Anova and Tukey's post hoc analysis: P34 vs. P42: p = 0.005∗; P34 vs P60: p = 0.0002∗; P34-P60: p = 0.072, not significant. G. Double immunofluorescence staining for NECAB1 (blue) and tdT using mCherry antibodies (red) in confocal images (single optical sections) showed that a subset of mCherry + neurons co-expressed NECAB1 (∼25 %). Images were captured with a 20X objective in a field of view of 1024× 1024 pixels, or 319 μm × 319 μm. Representative regions of the confocal images are shown. Bars = 10 μm. Control staining with secondary antibodies alone was negative. H. Double immunofluorescence staining for parvalbumin (PV; blue) and tdTomato (anti-dsRed, red) indicated no detectable co-localization in representative confocal images. Images were captured with a 10X objective at 1024× 1024 pixels, or 639 μm × 639 μm. Representative regions of confocal images are shown. Bars = 50 μm.

Article Snippet: Primary antibody solutions contained 1:100 mouse anti-VGLUT3 antibody (Synaptic Systems 135211, 1 mg/ml) and 1:100 rabbit anti-Math2/NeuroD6 antibody (Abcam 85824, 0.9 mg/ml); 1:500 mouse anti-Parvalbumin (PV) 3C9 antibody (Novus Biologicals NBP2-50038, 1 mg/ml) and 1:500 rabbit anti-VGLUT3 antibody (Synaptic Systems 135203, 1 mg/ml); or 1:2500 rabbit anti-NECAB1 antibody (Sigma HPA023629, 0.20 mg/ml).

Techniques: Expressing, Staining, Labeling, Control, Fluorescence, Microscopy, Double Immunofluorescence Staining

Journal: Current Research in Neurobiology

Article Title: Regulation of perisomatic synapses from cholecystokinin basket interneurons through NrCAM and Ankyrin B

doi: 10.1016/j.crneur.2025.100150

Figure Lengend Snippet: NrCAM expression in pyramidal neurons, CCK basket cells, and GFAP + astrocytes in mPFC (layer II/III) A. Double Immunofluorescence staining for NrCAM (blue) in Nex1Cre-ERT2: Ank2+/+; EGFP (WT) mice (P21) in PNs expressing eGFP (red). Confocal images captured at 63X (single optical section, 0.3 μm) showed colocalization of NrCAM on PN soma. Arrowheads point to NrCAM + processes in proximity to EGFP + PN soma. Control staining with secondary antibodies alone. Bars = 10 μm. B. Double immunofluorescence staining for NrCAM (blue) in tdT + CCK-BCs of Sncg-IRES-FlpO:tdT mice labeled with mCherry antibodies (red). Confocal images captured at 63X (single optical section, 0.3 μm) show NrCAM localization on CCK-BCs (arrowheads). Control with secondary antibodies alone. Bars = 10 μm. C. Double immunofluorescence staining for NrCAM (blue) and GFAP (red) in tdT + CCK-BCs of Sncg-IRES-FlpO:tdT mice. EVOS images captured at 20X show colocalization of NrCAM in GFAP + astrocytes. D. Double immunofluorescence staining for NrCAM (blue) and parvalbumin (PV) (red) in CCK-BCs of Sncg-IRES-FlpO:tdT mice in mPFC layer 2/3. EVOS images captured at 20X show colocalization of NrCAM in a subpopulation of PV + interneurons. Control staining with secondary antibodies alone was negative. NrCAM antibodies did not label cells in NrCAM-null mice (knockout, KO) as shown in mPFC layer 2/3. Bars = 10 μm.

Article Snippet: Primary antibody solutions contained 1:100 mouse anti-VGLUT3 antibody (Synaptic Systems 135211, 1 mg/ml) and 1:100 rabbit anti-Math2/NeuroD6 antibody (Abcam 85824, 0.9 mg/ml); 1:500 mouse anti-Parvalbumin (PV) 3C9 antibody (Novus Biologicals NBP2-50038, 1 mg/ml) and 1:500 rabbit anti-VGLUT3 antibody (Synaptic Systems 135203, 1 mg/ml); or 1:2500 rabbit anti-NECAB1 antibody (Sigma HPA023629, 0.20 mg/ml).

Techniques: Expressing, Double Immunofluorescence Staining, Control, Staining, Labeling, Knock-Out